cd163  (Bioss)

Journal: ACS Omega

Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

doi: 10.1021/acsomega.4c10118

Figure Lengend Snippet: M1 and M2 macrophage characterization by qPCR (a) and Flow Cytometry (b). The M1 phenotype is characterized by CD68 and CD80 gene expressions, while CD163, ARG1, and CD206 gene expressions are present in the M2 macrophage phenotype. All results are expressed as mean ± standard deviation (SD) from two independent experiments, each performed with a minimum of three replicates. Statistically significant differences are indicated by multiplicity-adjusted p -values: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Data were analyzed using one-way ANOVA followed by post hoc Dunnett’s correction.

Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

Techniques: Flow Cytometry, Standard Deviation

Journal: ACS Omega

Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

doi: 10.1021/acsomega.4c10118

Figure Lengend Snippet: Liver expression of AKT (a–c), TGFb (d–f), CD163 (g–i), CD86 (j–l), NFkB (m–o), IL-10 (p–r), and Pi3K (s–u) in Wistar rats submitted to alcoholic liver injury.

Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

Techniques: Expressing

Journal: ACS Omega

Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

doi: 10.1021/acsomega.4c10118

Figure Lengend Snippet: Liver expression of AKT (a), TGFb (b), CD163 (c), CD86 (d), NFkB (e), IL-10 (f), and Pi3K (g). All data are presented as mean ± SD. Fluorescence intensity data was generated by Zeiss Zen Lite software for each picture taken (10 images per subject, 5 animals per group, 400× magnification). Statistically significant differences are indicated as follows: * p < 0.05, ** p < 0.005, *** p < 0.001, and **** p < 0.0001; (ns) denotes no significant difference. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc correction for multiple comparisons.

Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

Techniques: Expressing, Fluorescence, Generated, Software

Journal: ACS Omega

Article Title: Gold Nanoparticles (AuNPs) Coadministered with a β-Blocker Prevent Liver Fibrosis Caused by Ethanol and Methamphetamine in Rats by Downregulating the Expression of M2 Macrophages

doi: 10.1021/acsomega.4c10118

Figure Lengend Snippet: Comparison between M1 (CD86) and M2 (CD163) immunofluorescence labeling in Wistar rats submitted to alcoholic liver injury. All data are presented as mean ± SD. Fluorescence intensity measurements were obtained using Zeiss Zen Lite software, with 10 images captured per subject, 5 animals per group, at 400× magnification. Statistically significant differences are denoted as * p < 0.05 and ** p < 0.005; (ns) indicates no significant difference. Data were analyzed using two-way ANOVA with Sidak’s post hoc correction.

Article Snippet: Fluorescently labeled antibodies, including CD163-FITC (200 ng; Bioss, bs-2527R-FITC) and CD68-PerCP (200 ng; Abcam, ab220509), were used for specific staining.

Techniques: Comparison, Immunofluorescence, Labeling, Fluorescence, Software